Optimizing laboratory production of Strelkovimermis spiculatus (Nematoda: Mermithidae) with a discussion of potential release strategies for mosquito biological control

► We determined the J2 concentration producing a maximum J4. ► Ratios between 4:1 and 7:1 J2/A. aegypti larvae are adequate to obtain an optimum mass of nematodes. ► J2 production was evaluated after J4 addition in cultures permanently flooded and desiccated. ► Permanent cultures produced a lower total number of J2. ► J2s and J4s would serve as bioregulator to temporary or permanent mosquito habitats.