کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4504575 | 1321099 | 2010 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of nonpathogenic Cadophora gregata, a potential biological control agent, concomitantly isolated from soil infested with Cadophora gregata f. sp. adzukicola, the cause of adzuki bean brown stem rot
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
We collected 555 isolates of Cadophora gregata from adzuki bean field soils in Hokkaido, Japan, from 1997 to 2000. To identify the brown stem rot (BSR) pathogen C. gregata f. sp. adzukicola, we screened these isolates for pathogenicity to adzuki beans. Of the isolates, all of which originated in Tokachi District, Hokkaido, Japan, 23 were avirulent to adzuki bean, soybean, or mung bean. However, polymerase chain reaction (PCR) with specific primers for C. gregata f. sp. adzukicola (BSRA1 and BSRA2) detected the specific identifying DNA fragment in these isolates, and cluster analysis with inter-simple sequence repeat markers showed that the isolates were phylogenetically closer to strains that are virulent to adzuki bean. Thus, we concluded that the isolates were nonpathogenic C. gregata. A few selected isolates of the nonpathogenic C. gregata were effective at reducing BSR in vivo and show potential for development as biological control agents.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biological Control - Volume 53, Issue 1, April 2010, Pages 112-121
Journal: Biological Control - Volume 53, Issue 1, April 2010, Pages 112-121
نویسندگان
Soichi Tanaka, Keiko Murayama, Norio Kondo, Seishi Akino,