کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4504694 | 1321106 | 2009 | 7 صفحه PDF | دانلود رایگان |
The present study investigated the inhibitory effect on the conidial germination of Venturia inaequalis by using living whole cells, extracellular protein fraction and individual proteins from the non-pathogenic antagonistic bacterium Pseudomonas fluorescens Bk3.The bacterial suspension of P. fluorescens Bk3 from growth in minimal medium showed up to 73% inhibition of conidial germination after 7 days of pre-incubation. Furthermore, this inhibitory effect could also be shown by the extra cellular protein fraction of P. fluorescens Bk3. The protein solution obtained from liquid culture in LB medium showed 100% inhibition of conidial germination after 1 day of pre-incubation. Since the solution contained at least 10 major proteins these proteins were extracted from the gel and subsequently re-natured for functional studies. After re-naturation individual proteins were applied on the conidia of V. inaequalis to see their impact on the conidial germination. Out of these 10 proteins three showed inhibitory effects (20–42%). De novo sequencing of these three proteins were carried out by ESI Q-ToF mass spectrometry and they were identified as an extracellular solute-binding protein, an extracellular alkaline metalloprotease and a peptidoglycan-associated lipoprotein. The proteolytic activity of the metalloprotease could also be confirmed with activity staining using casein as a substrate.
Journal: Biological Control - Volume 48, Issue 2, February 2009, Pages 133–139