کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4505256 1321135 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Insertional mutation of the rpoS gene contributes to alteration in biosynthesis of antifungal agents in Pseudomonas sp. M18
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Insertional mutation of the rpoS gene contributes to alteration in biosynthesis of antifungal agents in Pseudomonas sp. M18
چکیده انگلیسی

As a plant-beneficial rhizobacterium, Pseudomonas sp. M18 was isolated from the rhizosphere of watermelon in a Shanghai suburb. Two kinds of antifungal compounds, phenazine-1-carboxylic acid and pyoluteorin produced by Pseudomonas sp. M18, contribute to suppression of pathogenic fungi in agricultural soil. In order to examine how production of these antifungal agents is regulated, an rpoS gene with its flanking fragments was first cloned in strain M18. With the insertional inactivation of rpoS, the rpoS null mutant strain M18S was constructed, and it showed improved production of antifungal agents. In King’s medium B, phenazine-1-carboxylic acid reduced to nondetectable level, while pyoluteorin increased approximately 10-fold. In PPM medium, strain M18S produced less phenazine-1-carboxylic acid and much more pyoluteorin in comparison with wild-type strain M18. Complementation with the wild type ropS gene in trans restored wild-type phenotypes. Expression of the translational fusions phzA‘-’lacZ and pltA‘-’lacZ confirmed the effect of rpoS on phenazine-1-carboxylic acid and pyoluteorin biosynthetic operons. In in vitro plate assays, the mutant strain M18S inhibiting mycelial growth of Pythium aphanidermatum more than wild-type strain M18. Altogether, these results suggest that phenazine-1-carboxylic acid biosynthesis is positively regulated and pyoluteorin production negatively regulated by alternative sigma factor S in Pseudomonas sp. M18.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biological Control - Volume 39, Issue 2, November 2006, Pages 186–192
نویسندگان
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