کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4529290 | 1625948 | 2014 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Characterization of cadmium plasma membrane transport in gills of a mangrove crab Ucides cordatus Characterization of cadmium plasma membrane transport in gills of a mangrove crab Ucides cordatus](/preview/png/4529290.png)
• Cd2+ gill cell transport, a non-essential toxic metal, was characterized in a hypo-hyper-regulating mangrove crab Ucides cordatus.
• Cd2+ enter gill cells through Ca2+ channels and is dependent of intracellular Ca2+ levels.
• Route of entry in gill cells also involves a Cd2+/Ca2+ (2Na) exchanger.
• Cd transport depends on Na+/K+-ATPase and gill cell electrochemical gradient.
• Vanadate inhibits gill Cd2+ transport and ouabain increase gill Cd2+ transport.
Membrane pathway for intracellular cadmium (Cd2+) accumulation is not fully elucidated in many organisms and has not been studied in crab gill cells. To characterize membrane Cd2+ transport of anterior and posterior gill cells of Ucides cordatus, a hypo-hyper-regulating crab, a change in intracellular Cd2+ concentration under various experimental conditions was examined by using FluoZin, a fluorescent probe. The membrane Cd2+ transport was estimated by the augmentation of FluoZin fluorescence induced by extracellular application of CdCl2 and different inhibitors. Addition of extracellular calcium (Ca2+) to the cells affected little the fluorescence of FluoZin, confirming that Cd2+ was the main ion increasing intracellular fluorescence. Ca2+ channels blockers (nimodipine and verapamil) decreased Cd2+ influx as well as vanadate, a Ca2+-ATPase blocker. Chelating intracellular Ca2+ (BAPTA) decreased Cd2+ influx in gill cells, while increasing intracellular Ca2+ (caffeine) augmented Cd influx. Cd2+ and ATP added at different temporal conditions were not effective at increasing intracellular Cd2+ accumulation. Ouabain (Na+/K+-ATPase inhibitor) increased Cd2+ influx probably through a change in intracellular Na and/or a change in cell membrane potential. Routes of Cd2+ influx, a non-essential metal, through the gill cell plasma membrane of crabs are suggested.
Journal: Aquatic Toxicology - Volume 157, December 2014, Pages 21–29