کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4529374 | 1625957 | 2014 | 10 صفحه PDF | دانلود رایگان |

• Daphnia magna MnSOD (Dm-MnSOD) was identified and revealed MnSOD-family features.
• The expression of Dm-MnSOD decreased with increased developmental stages.
• Dm-MnSOD transcript was kinetically up-regulated by microcystin, nitrite and Cd.
• Response of SOD to ubiquitous waterborne pollutants in D. magna was elucidated.
• Dm-MnSOD gene is a potential biomarker indicating pollutants in the environment.
Superoxide dismutases (SODs) are metalloenzymes that represent one important line of defense against oxidative stress produced by reactive oxygen species in aerobic organisms. Generally, waterborne pollutants caused by irregular anthropogenic activities often result in oxidative damage in aquatic organisms. The aim of this study was to molecularly characterize the manganese superoxide dismutase gene (Dm-MnSOD) in the waterflea, Daphnia magna, and evaluate the mRNA expression patterns quantified by real-time PCR after exposure to three common waterborne pollutants (microcystin-LR, nitrite, and cadmium). The results showed that the full-length Dm-MnSOD sequence consists of 954 bp nucleotides, encoding 215 amino acids, showing well-conserved domains that are required for metal binding and several common characteristics, such as two MnSOD domains. The deduced amino acid sequence of Dm-MnSOD shared over 70% similarity with homologues from Bythograea thermydron, Dromia personata, Cancer pagurus, and Scylla paramamosain. Dm-MnSOD gene expression was up-regulated in response to exposure to the three chemicals tested. The overall results indicated that Dm-MnSOD gene is an inducible gene and potential biomarker indicating these pollutants in the environment.
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Journal: Aquatic Toxicology - Volume 148, March 2014, Pages 55–64