کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4545141 1626916 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Toxigenic algae and associated phycotoxins in two coastal embayments in the Ebro Delta (NW Mediterranean)
ترجمه فارسی عنوان
جلبک های توکسینیکی و فیتوتوکسین های مرتبط در دو جاذبه ساحلی در دلتا ابرو (دریای مدیترانه)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
چکیده انگلیسی


• Comprehensive surveillance of algal taxa in Ebro Delta bays by integrating methods.
• Next generation DNA sequencing brings attention to regionally new HAB taxa.
• Record of potentially toxic dinoflagellate genera new to Ebro Delta region.
• First significant concentration of domoic acid (DA) measured in Ebro Delta plankton.
• DA concentration does not coincide with cell abundance of Pseudo-nitzschia spp.

Harmful Algal Bloom (HAB) surveillance is complicated by high diversity of species and associated phycotoxins. Such species-level information on taxonomic affiliations and on cell abundance and toxin content is, however, crucial for effective monitoring, especially of aquaculture and fisheries areas. The aim addressed in this study was to determine putative HAB taxa and related phycotoxins in plankton from aquaculture sites in the Ebro Delta, NW Mediterranean. The comparative geographical distribution of potentially harmful plankton taxa was established by weekly field sampling throughout the water column during late spring–early summer over two years at key stations in Alfacs and Fangar embayments within the Ebro Delta. Core results included not only confirmed identification of HAB taxa that are common for the time period and geographical area, but also provided evidence of potentially new taxa. At least 25 HAB taxa were identified to species level, and an additional six genera were confirmed, by morphological criteria under light microscopy and/or by molecular genetics approaches involving qPCR and next generation DNA pyrosequencing. In particular, new insights were gained by the inclusion of molecular techniques, which focused attention on the HAB genera Alexandrium, Karlodinium, and Pseudo-nitzschia. Noteworthy is the discovery of Azadinium sp., a potentially new HAB species for this area, and Gymnodinium catenatum or Gymnodinium impudicum by means of light microscopy. In addition, significant amounts of the neurotoxin domoic acid (DA) were found for the first time in phytoplankton samples in the Ebro Delta. While the presence of the known DA-producing diatom genus Pseudo-nitzschia was confirmed in corresponding samples, the maximal toxin concentration did not coincide with highest cell abundances of the genus and the responsible species could not be identified. Combined findings of microscopic and molecular detection approaches underline the need for a synoptic strategy for HAB monitoring, which integrates the respective advantages and compensates for limitations of individual methods.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Harmful Algae - Volume 55, May 2016, Pages 191–201
نویسندگان
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