کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4545240 1626927 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Morphology, phylogeny and novel chemical compounds from Coolia malayensis (Dinophyceae) from Okinawa, Japan
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Morphology, phylogeny and novel chemical compounds from Coolia malayensis (Dinophyceae) from Okinawa, Japan
چکیده انگلیسی


• Coolia malayensis isolated and mass-cultivated from a reef on Okinawa, Japan.
• Chemical analyses confirmed the production of yessotoxin analogs in this strain.
• High-resolution mass spectrometry elucidated three novel chemical formulas.

Marine benthic dinoflagellates within the genus Coolia have been reported to produce natural products, some of which are known to be toxic (i.e., cooliatoxin). To date, five species of Coolia have been reported in tropical and temperate waters around the world; however, very few studies have combined detailed morphological and molecular data with chemical analyses. In this study, a clonal culture of Coolia malayensis was isolated and mass cultivated from a coral reef on the island of Okinawa, Japan. Analysis of the thecal plate morphology and molecular phylogeny from 28S rDNA strongly supported the close relationship between this new isolate of C. malayensis from Okinawa and other isolates of C. malayensis from around the world. Following methanol extraction of 250 L of mass culture, chemical analyses using NanoLiquid chromatography mass spectrometry revealed the mass profiles of water-soluble and ethyl acetate-soluble parts. High-resolution mass spectrometry derived the molecular formulas of three novel disulphated polyether analogs of yessotoxin (C56H78O18S2 1102.4 (Compound 1), C57H80O18S2 1116.4 (Compound 2), and C57H78O19S2 1130.4 (Compound 3)); two potential homologous compounds (Compounds 4 and 5) were also observed on the high-resolution mass, albeit with low signal intensity. The five compounds in the C. malayensis from Okinawa are composed of less oxygen, compared to cooliatoxin and other analogs of yessotoxin, suggesting the metabolites produced by C. malayensis are unique to those previously reported from other strains of Coolia.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Harmful Algae - Volume 44, April 2015, Pages 8–19
نویسندگان
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