کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4545531 1626952 2012 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transcriptomic response of the toxic prymnesiophyte Prymnesium parvum (N. Carter) to phosphorus and nitrogen starvation
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Transcriptomic response of the toxic prymnesiophyte Prymnesium parvum (N. Carter) to phosphorus and nitrogen starvation
چکیده انگلیسی

The ichthyotoxic and mixotrophic prymnesiophyte Prymnesium parvum is known to produce dense virtually monospecific blooms in marine coastal, brackish, and inshore waters. Fish-killing Pyrmnesium blooms are often associated with macronutrient imbalanced conditions based upon shifts in ambient nitrogen (N):phosphorus (P) ratios. We therefore investigated nutrient-dependent cellular acclimation mechanisms of this microalga based upon construction of a normalized expressed sequence tag (EST) library. We then profiled the transcriptome of P. parvum under nutrient-replete conditions as well as under nitrogen (N) and phosphorus (P) limitation via microarray analyses. Twenty-three genes putatively involved in acclimation to low nutrient levels were identified, among them three phosphate transporters, which were highly upregulated under P-starvation. In contrast, the expression of genes involved in transport and acquisition of ammonium or nitrate/nitrite was unaltered in N-starved cells. We propose that genes upregulated under P- or N-starvation lend themselves as potential tools to monitor nutrient limitation effects at the cellular level and indirectly the potential for initiation and maintenance of toxic blooms of P. parvum.


► We sequenced a normalized cDNA library of the toxic prymnesiophyte Prymnesium parvum.
► We investigated the effect of nitrogen or phosphorus starvation on the toxicity and gene expression of P. parvum.
► We identified genes which can be used as sensitive indicators for putative bloom events.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Harmful Algae - Volume 18, June 2012, Pages 1–15
نویسندگان
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