کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4545939 | 1327479 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of several harmful algal species by sandwich hybridization integrated with a nuclease protection assay
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The frequent occurrence of harmful algal blooms (HABs) is a pressing topic in marine research. An integrated sandwich hybridization and nuclease protection assay was established to qualitatively and quantitatively detect 12 harmful algal species. This method demonstrated good reliability, specificity and accuracy for analyzing samples from individual and mixed cultures, as well as field collection, and cell volumes were positively correlated to the slopes of calibration curves. The lowest quantitative detection limits were those concentrations observed during blooms; thus, this technique provides an efficient alternative to microscopy for rapid identification and quantitation of harmful algal species and could be routinely used to monitor phytoplankton in field surveys.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Harmful Algae - Volume 8, Issue 5, June 2009, Pages 651-657
Journal: Harmful Algae - Volume 8, Issue 5, June 2009, Pages 651-657
نویسندگان
Yu Zhen, Tiezhu Mi, Zhigang Yu,