Evaluation of the mitochondrial system in the gonad-digestive gland complex of Biomphalaria glabrata (Mollusca, Gastropoda) after infection by Echinostoma paraensei (Trematoda, Echinostomatidae)

• Echinostoma paraensei changes the aerobic oxidative metabolism of Biomphalaria glabrata.
• The infection suppresses mitochondrial phosphorylation state of B. glabrata.
• The infection by E. paraensei change mitochondrial different states of B. glabrata.
• The infection alters mitochondrial 3u state of B. glabrata.

The effect of infection by Echinostoma paraensei on the mitochondrial physiology of Biomphalaria glabrata was investigated after exposure to 50 miracidia. The snails were dissected one, two, three and four weeks after infection for collection and mechanical permeabilization of the gonad-digestive gland (DGG) complex. The results obtained indicate that prepatent infection by this echinostomatid fluke significantly suppresses the phosphorylation state (respiratory state 3) and basal oxygen consumption of B. glabrata, demonstrating that the infection reduces the ability of the intermediate host to carry out aerobic oxidative reactions. Additionally, relevant variations related to the uncoupled mitochondrial (state 3u) of B. glabrata infected by E. paraensei were observed. Four weeks after exposure, a significant reduction in mitochondrial oxygen consumption after addition of ADP (3.68 ± 0.26 pmol O2/mg proteins) was observed in the infected snails in comparison with the respective control group (5.14 ± 0.25). In the uncoupled state, the infected snails consumed about 62% less oxygen than the infected snails (7.87 ± 0.84 pmol O2/mg proteins) in the same period. These results demonstrate a reduction in oxidative decarboxylation rate of the tricarboxylic acid cycle and faster anaerobic degradation of carbohydrates in the infected snails. The possible mechanisms that explain this new metabolic condition in the infected organisms are discussed.

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