Large scale patterns of abundance and distribution of parasites in Mexican bumblebees

• We screened wild Mexican bumblebees for the presence of Crithidia and Nosema.
• Based on genetic divergence, we report the presence of a new Crithidia species.
• We suggest the name Crithidia mexicana for this newly discovered organism.
• Based on microsatellite data, we discovered an isolated population of C. expoeki.
• Our study sheds light on the evolutionary/phylo-geographic history of Crithidia.

Bumblebees are highly valued for their pollination services in natural ecosystems as well as for agricultural crops. These precious pollinators are known to be declining worldwide, and one major factor contributing to this decline are infections by parasites.Knowledge about parasites in wild bumblebee populations is thus of paramount importance for conservation purposes. We here report the geographical distribution of Crithidia and Nosema, two common parasites of bumblebees, in a yet poorly investigated country: Mexico.Based on sequence divergence of the Cytochrome b and Glycosomal glyceraldehyde phosphate deshydrogenase (gGPDAH) genes, we discovered the presence of a new Crithidia species, which is mainly distributed in the southern half of the country. It is placed by Bayesian inference as a sister species to C. bombi. We suggest the name Crithidia mexicana for this newly discovered organism.A population of C. expoeki was encountered concentrated on the flanks of the dormant volcanic mountain, Iztaccihuatl, and microsatellite data showed evidence of a bottleneck in this population.This study is the first to provide a large-scale insight into the health status of endemic bumblebees in Mexico, based on a large sample size (n = 3,285 bees examined) over a variety of host species and habitats.