Overexpression of Bm65 correlates with reduced susceptibility to inactivation by UV light

• Bm65 was found to mainly accumulate within the nucleus.
• Bm65 effectively increases the survival rate of E. coli after UV treatment.
• Overexpression of Bm65 reduced the UV inactivation of the budded virus and might play a role in viral DNA repair.

Ultraviolet (UV) light is one of the factors that causes baculovirus inactivation. However, little is known about the response of baculoviruses to UV light. In the present study, Bombyx mori nucleopolyhedrovirus (BmNPV) orf 65 (Bm65), the homolog of Autographa californica nucleopolyhedrovirus orf 79 (Ac79), a predicted endonuclease, was analyzed. Preliminary results indicated that Bm65 mainly accumulated within the nucleus and could improve the survival rate of Escherichia coli (E. coli) and BmNPV BVs after UV radiation, suggesting that Bm65 was involved in the repair of UV-induced DNA damage.

Overexpression of Bm65 reduced the UV inactivation of the budded virus.Figure optionsDownload as PowerPoint slide