White spot syndrome virus VP12 interacts with adenine nucleotide translocase of Litopenaeus vannamei

• White spot syndrome virus VP12 interacts with LvANT with dose-dependent manner.
• LvANT was commonly found in all the tissues detected.
• LvANT was colocalized with mitochondrion in shrimp hemocytes.
• Injection of LvANT antiserum decreased the cumulative mortality of shrimp challenged by WSSV.

White spot syndrome virus VP12 contains cell attachment motif RGD which is considered to be critical for host cell binding. Until now, the function of this protein remains undefined. In this study, we explored the interaction of VP12 with host cells. A new shrimp protein (adenine nucleotide translocase of Litopenaeus vannamei, LvANT) is selected by far-western overlay assay. Tissue distribution of adenine nucleotide translocase mRNA showed that it was commonly spread in all the tissues detected. Cellular localization of LvANT in shrimp hemocytes showed that it was primarily located in the cytoplasm of hemocytes and colocalized with mitochondria. ELISA and far-western blot assay confirmed that VP12 interacted with LvANT. In vivo neutralization assay showed that anti-LvANT antibody can significantly reduce the mortality of shrimp challenged by WSSV at 48 h post-treatment. Our results collectively showed that VP12 is involved in host cell binding via interaction with adenine nucleotide translocase.

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