کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4557802 | 1628235 | 2014 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A screening of five Bacillus thuringiensis Vip3A proteins for their activity against lepidopteran pests A screening of five Bacillus thuringiensis Vip3A proteins for their activity against lepidopteran pests](/preview/png/4557802.png)
• Five Vip3A proteins were tested against eight lepidopterans.
• Vip3Aa, Vip3Ae and Vip3Af were, in general, the most toxic proteins.
• Vip3Ab was little toxic; Vip3Ad was found nontoxic to all species tested.
• No major differences were found when comparing protoxins vs. trypsin-activated toxins.
• Ostrinia nubilalis only showed some susceptibility to Vip3Af.
Five Bacillus thuringiensis Vip3A proteins (Vip3Aa, Vip3Ab, Vip3Ad, Vip3Ae and Vip3Af) and their corresponding trypsin-activated toxins were tested for their toxicity against eight lepidopteran pests: Agrotis ipsilon, Helicoverpa armigera, Mamestra brassicae, Spodoptera exigua, Spodoptera frugiperda, Spodoptera littoralis, Ostrinia nubilalis and Lobesia botrana. Toxicity was first tested at a high dose at 7 and 10 days. No major differences were found when comparing protoxins vs. trypsin-activated toxins. The proteins that were active against most of the insect species were Vip3Aa, Vip3Ae and Vip3Af, followed by Vip3Ab. Vip3Ad was non-toxic to any of the species tested. Considering the results by insect species, A. ipsilon, S. frugiperda and S. littoralis were susceptible to Vip3Aa, Vip3Ab, Vip3Ae and Vip3Af; S. exigua was susceptible to Vip3Aa and Vip3Ae, and moderately susceptible to Vip3Ab; M. brassicae and L. botrana were susceptible to Vip3Aa, Vip3Ae and Vip3Af; H. armigera was moderately susceptible to Vip3Aa, Vip3Ae and Vip3Af, and O. nubilalis was tolerant to all Vip3 proteins tested, although it showed some susceptibility to Vip3Af. The results obtained will help to design new combinations of insecticidal protein genes in transgenic crops or in recombinant bacteria for the control of insect pests.
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Journal: Journal of Invertebrate Pathology - Volume 117, March 2014, Pages 51–55