کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4558354 | 1329936 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Selection of improved Beauveria bassiana (Bals.) Vuill. strains based on 2-deoxy-d-glucose resistance and physiological analysis
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
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چکیده انگلیسی
A series of 2-deoxy-d-glucose resistant mutants was obtained from wild type Beauveria bassiana 88 (Bb 88) by UV irradiation. Five mutants were characterized on Sabouraud Dextrose Agar and Chitin Agar for both radial extension rate (Vr) and specific growth rate (μ). These values were obtained after adjusting morphometric data to a mathematical model used for filamentous fungi. Additionally, the protease and lipase potency index, conidial size, viability, and production levels were analyzed. The highest values for those physiological measurements were obtained by mutant 882.5 which, relative to Bb 88, showed a 30% reduction in half-life (LT50) on Sphenarium purpurascens, 70% on Acheta domesticus, and 71% on Tenebrio molitor larvae and adults. The half lethal concentration (LC50) on T. molitor larvae was 2.8 Ã 105 conidia/mL (con/mL) and 1.5 Ã 106 con/mL, respectively, for mutant 882.5 and Bb 88. This demonstrates that mutant 882.5 is more virulent, with up to an 80% reduction in LC50. This work provides a convenient method for improving strains to be used in biocontrol as a suitable alternative to transgenic constructs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Invertebrate Pathology - Volume 101, Issue 3, July 2009, Pages 222-227
Journal: Journal of Invertebrate Pathology - Volume 101, Issue 3, July 2009, Pages 222-227
نویسندگان
M. Robledo-Monterrubio, R. Alatorre-Rosas, G. Viniegra-González, O. Loera,