| کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
|---|---|---|---|---|
| 4565856 | 1331087 | 2016 | 6 صفحه PDF | دانلود رایگان |
کلمات کلیدی
1.مقدمه
2- مواد و روش ها
1-2- مواد گیاهی
2-2- آنالیز بیوانفورماتیک MdCIbHLH1
3-2- آنالیز بیان MdCIbHLH1 در دوره های مختلف خواب
4-2- تراریختی آرابیدوپسیس تالیانا
3- نتایج
3-1- آنالیز بیوانفورماتیکی پروتئین MdCIbHLH1
2-3- دمین عملکردی حفاظت شده و آنالیز درخت فیلوژنتیکی MdCIbHLH1
3-3- آنالیز بیان MdCIbHLH1 طی دوره شکست خواب جوانه گل
3-4- آنالیز بیان MdCIbHLH1 طی فرآیند استریفیکاسیون دانه
5-3- شناسایی فنوتیپ بیش بیان MdCIbHLH1 هترولوکوس در آرابیدوپسیس
4- بحث
A bHLH transcription factor that is induced by low temperature was found in apple (Malus × domestica Borkh.). To understand the sequence characteristics of the gene, bioinformatics analysis was performed. Furthermore, gene expression patterns of the laminated apple seeds and lateral flower buds were analyzed during the period of dormancy release with semi-quantitative RT-PCR. Based on secondary structure predictions, the results showed that the MdCIbHLH1 protein structure mainly included α-helix and random coil, while β-sheet and extended strand content was less. Semi-quantitative RT-PCR analysis showed that the expression patterns of MdCIbHLH1 were similar in laminated apple seeds and lateral flower buds during the period of dormancy release. Before dormancy release, expression levels of MdCIbHLH1 were high and gradually decreased during the period of dormancy release. These results indicated that MdCIbHLH1 might play an important role during dormancy release in apple seeds and apple buds.
Journal: Horticultural Plant Journal - Volume 2, Issue 2, March 2016, Pages 61–66