کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4581636 | 1333711 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: An Attempt to Quantify Cu-Resistant Microorganisms in a Paddy Soil from Jiaxing, China An Attempt to Quantify Cu-Resistant Microorganisms in a Paddy Soil from Jiaxing, China](/preview/png/4581636.png)
Understanding the mechanisms of Cu pollution-induced community tolerance (PICT) in soil requires the characterization of Cu-resistant microorganisms at a community level using modern molecular tools. A primer pair (copAF2010 (5′-TGCAC CTGAC VGGSC AYAT-3′)/copAR2333 (5′-GVACT TCRCG GAACA TRCC-3′)) tentatively targeting Pseudomonas-like Cu-resistant microorganisms was designed in this study. The specification of the primers was tested through conventional polymerase chain reaction (PCR) and the construction of a Pseudomonas-like copA gene fragment library, and then the primers were used to quantify the Cu-resistant microorganisms using quantitative PCR technique. A significant increase of Cu-resistant microorganisms targeted by the primers was observed in a paddy soil from Jiaxing, China which has been exposed to one-year Cu contamination. The results provided direct evidence for Cu PICT in the soil, and the quantification method developed in this study has the potential to be used as a molecular assay for soil Cu pollution.
Journal: Pedosphere - Volume 22, Issue 2, April 2012, Pages 201-205