Genome engineering in human pluripotent stem cells

- Current status, challenges and outlook for CRISPR/Cas9 genome engineering in hPSCs.
- Applications in investigating human biology, disease and therapy.
- Novel applications of dCas9-based fusions.

Human pluripotent stem cells (hPSCs) hold great promise for modeling and recapitulation of human biological processes. Coupled with the advent of genome engineering tools, specifically the CRISPR-Cas9 systems, hPSCs have opened a multitude of possibilities in modeling human biology and creating novel cellular therapies. Here, we review the fundamentals of both technologies, and the wide-ranging present and future applications of genome engineering in hPSCs.