کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4765093 | 1423854 | 2017 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Data ArticleData for automated, high-throughput microscopy analysis of intracellular bacterial colonies using spot detection Data ArticleData for automated, high-throughput microscopy analysis of intracellular bacterial colonies using spot detection](/preview/png/4765093.png)
Quantification of intracellular bacterial colonies is useful in strategies directed against bacterial attachment, subsequent cellular invasion and intracellular proliferation. An automated, high-throughput microscopy-method was established to quantify the number and size of intracellular bacterial colonies in infected host cells (Detection and quantification of intracellular bacterial colonies by automated, high-throughput microscopy, Ernstsen et al., 2017 [1]). The infected cells were imaged with a 10Ã objective and number of intracellular bacterial colonies, their size distribution and the number of cell nuclei were automatically quantified using a spot detection-tool. The spot detection-output was exported to Excel, where data analysis was performed. In this article, micrographs and spot detection data are made available to facilitate implementation of the method.
Journal: Data in Brief - Volume 14, October 2017, Pages 643-647