کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4765736 | 1423887 | 2018 | 8 صفحه PDF | دانلود رایگان |
- A class of DCM derivatives with large 2PA cross-section at NIR range were synthesized.
- PCM was successfully applied for labelling cancer cells with minimal autofluorescence.
- PCM was successfully utilized to labelling zebrafish with deeper penetration (>300 μm).
In vitro and in vivo fluorescence imaging of cells and animals plays a significant role in studying biological systems. However, autofluorescence becomes a serious issue in vivo imaging under excitation at UVâvis wavelengths where biological molecules cause interference with the chromophore. In order to address this issue, a novel class of dicyanomethylene-4H-chromene (DCM) derivatives (PCM, PCV, BPCM and BPCV) that can be excited at â¼900 nm under two-photon excitation conditions and emit in the red wavelength region (â¥600 nm) have been developed. Particularly, one of the DCM derivatives (PCM) shows several advantages, including remarkable two-photon absorption (2PA) properties in the near-infrared (NIR) region, superior photostability, large Stokes-shift and pronounced solvent-sensitive emission behavior, which is favorable for in vivo fluorescence labelling with a high signal-to-noise ratio.
A new DCM derivative (PCM) with remarkable two-photon absorption (2PA) properties was successfully applied for labelling cancer cells and zebrafish with minimal autofluorescence and deeper penetration (>300 μm).241
Journal: Dyes and Pigments - Volume 148, January 2018, Pages 429-436