Comparison of antibacterial and antifungal activity of 5-amino-2-mercapto benzimidazole and functionalized Ag3O4 nanoparticles

The work focused on the antibacterial and antifungal effect of 5-amino-2-mercapto benzimidazole (AMB), non-functionalized and functionalized Ag3O4 nanoparticles. The synthesized non-functionalized (n-Ag3O4) and functionalized (f-Ag3O4) nanoparticles particle sizes measured 18.72 and 29.11 nm respectively. The XRD results of samples exhibit shift in 2θ values of most prominent peaks due to surface functionalization of Ag3O4 nanoparticles with AMB. The surface functionalization of AMB on Ag3O4 nanoparticles further studied with FT-IR studies, which show the effective binding site is azomethine nitrogen evidenced from the shift of bands at 1635 and 1615 cm−1 in n-Ag3O4 nanoparticles to lower frequency region at 1629 and 1597 cm−1 in f-Ag3O4 nanoparticles. The functionalization of Ag3O4 nanoparticles and surface morphology further confirmed with HR-SEM with EDAX results. The antimicrobial effect of the non-functionalized and functionalized Ag3O4 nanoparticles dispersed in water was investigated. Antimicrobial activities were evaluated against bacterial strain of Pseudomonas aeruginosa, Staphylococcus aureus, and fungi Aspergillus niger used in this work. The n-Ag3O4 nanoparticles exhibit excellent antibacterial and antifungal effect compared to f-Ag3O4 nanoparticles and AMB. The decreased effect of f-Ag3O4 nanoparticles is might be due to increase in particle size and the interaction of silver oxide nanoparticles with bacteria membrane and intracellular proteins decreases with functionalization.

Figure optionsDownload as PowerPoint slide