کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
490787 | 717713 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of pH and Temperature on Bacillus sp. R2 Chitinase Activity and Stability
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی کامپیوتر
علوم کامپیوتر (عمومی)
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چکیده انگلیسی
The purified chitinase of Bacillus sp. R2 showed a molecular weight of 41.68 KDa, an optimal pH and temperature of 7.5 and 40 °C respectively. The enzyme was stable between pH 7 to 8 but it lost 57% and 62% of its initial activity at the pH 6 and 9 respectively. The purified chitinase also exhibited complete heat stability for 1 h at 40 °C and retained above 50% and 30% of its original activity after 30 min heating at 50 °C and 60 °C respectively. The chitinase activation energy for chitin hydrolysis was 12.93 Kcal/mol. To the best of our knowledge, this is the first study about marine chitinase characterization from novel gram variable Bacillus isolated from the Red sea.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Procedia Technology - Volume 22, 2016, Pages 471-477
Journal: Procedia Technology - Volume 22, 2016, Pages 471-477