Research paperSimple and sensitive adsorptive stripping voltammetric assay of granisetron from its dosage form by platinum nanoparticles modified electrodes

- Platinum particle modified glassy carbon electrode was developed by adsorptive stripping square wave voltammetry.
- The developed method was successfully applied to the determination of the drug in injectable dosage form with good recoveries.
- Detection limit of 1.95 nM was obtained at Platinum particle modified glassy carbon electrode for Granisetron.
- The possible electrooxidation pathway and the number of transferred electrons were obtained as 2e− and 1H+.

Base to the interfacial adsorptive nature of granisetron (GRN) on platinum particles modified glassy carbon electrode (PtPs/GCE). A sensitive analytical method for trace analysis of GRN based on the adsorptive stripping technique has been developed. The application of a square wave mode for the stripping of adsorbed GRN has proved to be much more sensitive compared to differential pulse scan. A study of the variation in the peak current with solution variables such as pH, concentration of granisetron, possible interference, and instrumental variables, such as accumulation time and accumulation potential, has resulted in the optimization of the oxidation signal for analytical purposes. Under the optimized conditions, the square wave peak current showed two linear ranges of GRN concentration: 0.01-0.5 μM and 0.5-25 μM with correlation coefficients of 0.997 and 0.998, respectively. Nanomolar detection limit was obtained as 1.95 nM for the first linear range. Possible interferences by substances usually present in the pharmaceutical formulations were also evaluated. The proposed electrochemical procedure was successfully applied for the determination of GRN in pharmaceutical injection samples (SETRON® 3 mg/3 mL) with average recovery of 100.30%.