Gold-palladium nanoparticles aided electrochemically reduced graphene oxide sensor for the simultaneous estimation of lomefloxacin and amoxicillin

- Gold-Palladium nanocomposite has been fabricated using reduced graphene oxide (rGO).
- The fabricated nanocomposite has been characterized using cyclic voltammetry, FE-SEM, EDX, TEM, Raman and EIS.
- The modified sensor has been used for the individual or simultaneous determination of lomefloxacin (LMF) and amoxicillin (AMX).
- High sensitivity and low detection limit have been observed for the both the antibiotics.
- No substantial interference is observed due to the metabolites present in urine.

A gold-palladium nanoparticles decorated electrochemically reduced graphene oxide (AuNP-PdNP-ErGO) modified, glassy carbon sensor has been developed for the individual and simultaneous determination of lomefloxacin (LMF) and amoxicillin (AMX). A new sensing platform exploiting the beneficial interaction of gold, palladium and ErGO has been prepared involving a one-step electrochemical process, and is characterized using Field Emission Scanning Electron Microscopy, elemental mapping, Transmission Electron Microscopy, Raman, X-ray diffraction and Electrochemical Impedance Spectroscopy. The calibration curves for LMF and AMX have been constructed using square wave voltammetry and exhibited a linear response in the concentration range of 4-500 μM and 30-350 μM respectively. The sensitivity and limit of detection were 0.0759 μA/μM and 81 nM, 0.0376 μA/μM and 9 μM for LMF and AMX respectively. The proposed protocol was successfully applied for testing the presence of LMF and AMX in the complex matrix like urine and in the solutions containing excess of potentially interfering substances like ascorbic acid, uric acid, hypoxanthine etc.

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