کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5009645 | 1462051 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fluorescence “turn on-off” detection of heparin and heparinase I based on the near-infrared emission polyethyleneimine capped Ag2S quantum dots
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A novel fluorescence “turn on-off” method for sensitive and selective detection of heparin and heparinase I was developed based on near infrared Polyethyleneimine Capped Ag2S Quantum Dots (PEI-Ag2S QDs). The fluorescence “turn-on” arose from aggregation-induced emission enhancement (AIEE) characteristics of PEI-Ag2SQDs induced by electrostatic interaction between PEI-Ag2SQDs and heparin. Heparinase I, which can specifically hydrolyze heparin into short fragments would de-aggregate PEI-Ag2S QDs/heparin system and lead to fluorescence “turn-off”. Under optimal conditions described, the fluorescence enhancement at 685 nm was linearly related to the concentration of heparin and heparinase I in the range of 0.069-2.275 μM and 0-103 ng mLâ1 with the relative correlation coefficient of R2 = 0.997 and 0.99 and detection limit as low as 6 nM and 0.5 ng mLâ1, respectively. The activity of the heparinase I was further investigated using steady-state kinetic for the reaction. In ddition, the proposed method was successfully utilized in serum samples, which presaged more opportunities for application in related biochemical and biomedical researches.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 240, March 2017, Pages 863-869
Journal: Sensors and Actuators B: Chemical - Volume 240, March 2017, Pages 863-869
نویسندگان
Dan Yan, Yu He, Yili Ge, Gongwu Song,