کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5009659 | 1462049 | 2017 | 8 صفحه PDF | دانلود رایگان |
- Paper-based laser induced fluorescence immunodevice for alpha fetoprotein detection was developed.
- A homemade laser induced fluorescence device with two optical fibres was adopted.
- CdTe QDs embedded silica nanoparticles with the signal enhancement function was assembled on the signal antibody.
- AFP was sensitively detected with the detection limit of 0.4Â pg/mL.
Paper-based laser induced fluorescence immunodevice for alpha fetoprotein (AFP) detection was developed in this work by using CdTe quantum dots (QDs) embedded silica nanoparticles as the signal enhancement label. A homemade laser induced fluorescence device with two optical fibres was used here for the fluorescence detection. Laser issued by a semiconductor laser light source (405Â nm, 1Â mW) was exposed to the paper-based chip with very lower background through an optical fiber and the fluorescence (542Â nm) induced by laser was collected to a photomultiplier tube (PMT) through a glass fiber. The paper-based chip was fabricated by the wax-printing method and the antibody can be directly immobilized on the plasma treated paper surface. Then CdTe QDs embedded silica nanoparticles with the signal enhancement function was labeled on the signal antibody. After a sandwich-type immunoreaction, AFP was linearly detected in the range from 0.001Â ng/mL to 20.0Â ng/mL with a detection limit of 0.4Â pg/mL. Laser induced fluorescence with the homemade device was for the first time applied on the paper-based chip with very higher sensitivity. What's more, the proposed strategy opened a promising platform for sensitive monitoring of cancer biomarkers in biological analysis and medical diagnostics.
123
Journal: Sensors and Actuators B: Chemical - Volume 242, April 2017, Pages 87-94