Optical biosensing using newly synthesized metal salphen complexes: A potential DNA diagnostic tool

A solid-state DNA optode sensor has been developed based on the employment of glutaraldehyde (GD)-activated aminated silica nanoparticles (SiO2NPs-NH2) DNA carrier matrix, and the newly synthesized yellowish N,N'-bis-5-(hydroxysalicylidene)phenylene-diamine-zinc(II) (i.e. the Zn salphen complex) oligonucleotide label. Some three Schiff base complexes with three different transition metal ions (Zn2+, Ni2+, Cu2+) were synthesized through a simple one-pot reaction (5 h), and characterized by their physical and chemical properties based on FTIR, NMR and ESI-MS analyses. Due to the square planar geometry and aromatic ring structured characteristics of the metal complex intercalators, they could intercalate between DNA bases via π-π stacking interaction, and rendered a yellowish pink hue on the DNA biosensor surface. Reflectometric optimization revealed high selectivity of the nanosilica-based DNA biosensor in the detection of dengue serotype 2 DNA, which capable of discriminating even a single nucleotide mismatch. The optical DNA biosensor demonstrated a linear reflectance response between 1.0 × 10−15 M and 1.0 × 10−11 M cDNA (R2 = 0.9975) with a fast DNA hybridization time of 30 min and a limit of detectable (LOD) DNA concentration as low as 1 zM. In addition, this biosensor showed high shelf life stability with a 20-day operational duration and reusable for five consecutive DNA testings. The proposed optical DNA biosensor offers biosensing performance far superior to that of previously reported electrochemical DNA biosensor for early diagnosis of dengue infection with respect to dynamic linear range, LOD and response time.