کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5009723 | 1462049 | 2017 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A DNA biosensor based on kappa-carrageenan-polypyrrole-gold nanoparticles composite for gender determination of Arowana fish (Scleropages formosus)
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The preparation and characterization of a DNA biosensor based on kappa-carrageenan-polypyrrole-gold nanoparticles (KC-PPy-AuNPs) nano-biocomposite is reported to demonstrate an application for gender classification of Arowana fish. Immobilization of the thiol modified Arowana fish ssDNA probe sequence was successfully carried out via covalent attachment to the gold (Au) on the surface of the nano-biocomposite membrane. Under optimum conditions, the DNA biosensor showed a short immobilization and hybridization time with high sensitivity for monitoring the target DNA sequence. The hybridization with the target DNA was determined by monitoring the redox current of anthraquinone-2-sulfonic acid monohydrate sodium salt (AQMS) using differential pulse voltammetry (DPV). The DNA hybridization response gradually increased with increasing concentrations of target DNA from 5Â ÃÂ 10â18 to 5Â ÃÂ 10â12Â M and the limit of detection was below 5Â ÃÂ 10â18Â M. The application of the DNA biosensor for gender classification of Arowana fish was demonstrated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 242, April 2017, Pages 616-624
Journal: Sensors and Actuators B: Chemical - Volume 242, April 2017, Pages 616-624
نویسندگان
Chakavak Esmaeili, Lee Yook Heng, Chew Poh Chiang, Zulkafli A. Rashid, Eka Safitri, Radha Swathe Priya Malon Marugan,