کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5009862 | 1462046 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Colorimetric sensor array for proteins discrimination based on the tunable peroxidase-like activity of AuNPs-DNA conjugates
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Colorimetric sensor array for proteins discrimination based on the tunable peroxidase-like activity of AuNPs-DNA conjugates Colorimetric sensor array for proteins discrimination based on the tunable peroxidase-like activity of AuNPs-DNA conjugates](/preview/png/5009862.png)
چکیده انگلیسی
We proposed a colorimetric sensor array for proteins discrimination based on the tunable catalytic activity of AuNPs-DNA conjugates. The peroxidase-like activity of AuNPs could be enhanced by the attachment of single-stranded oligonucleotides on its surface, resulting in highly sensitive colorimetric signal by catalyzing the oxidization of TMB. In the presence of different proteins, the AuNPs-DNA conjugates showed tunable catalytic activity, inducing differential colorimetric response. At the same time, the same protein could tune the catalytic activity of AuNPs decorated by different DNA with different degree. The fingerprint response generated from the sensor array was used for proteins discrimination. Compared to the colorimetric sensor array based on salt-induced AuNPs aggregation, this strategy exhibited much improved discrimination power for proteins at the low concentration. Seven proteins at the concentration of 10Â nM could be successfully identified and proteins spiked in human urine at the final concentration of 100Â nM could also be well distinguished. The discrimination accuracy of unknown samples was all 100% for these experiments. The current colorimetric array demonstrated its potential for highly sensitive diagnostic applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 245, June 2017, Pages 66-73
Journal: Sensors and Actuators B: Chemical - Volume 245, June 2017, Pages 66-73
نویسندگان
Jiaoe Yang, Yuexiang Lu, Liang Ao, Feiyang Wang, Wenjie Jing, Sichun Zhang, Yueying Liu,