کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5131528 | 1491316 | 2017 | 8 صفحه PDF | دانلود رایگان |
In this work, three-dimensional (3D) hyperbranched TiO2 nanorod arrays were synthesized and used to fabricate dopamine sensitized photoelectrochemical (PEC) biosensor. To increase the lifetime of charge carriers and enhance the photocurrent responses signal, a delicate signal amplification strategy by introducing dopamine (DA) as sensitizer was developed. The dopamine sensitized TiO2 can shorten the carrier diffusion distance, improve light harvesting efficiency and charge collection efficiency, which results in performance improvement of the as-obtained PEC sensor. This proposed biosensor for determination of neuron specific enolase (NSE) demonstrated a good linear relationship range from 0.1 ng mLâ1 to 1000 ng mLâ1 with a detection limit of 0.05 ngmLâ1 (S/N = 3). In addition, the as-prepared immunosensor exhibits excellent selectivity, stability and reproducibility, which could be extended to other label-free sensing fields. Therefore, this proposed method may also provide potential applications for the clinical examination.
Journal: Analytical Biochemistry - Volume 531, 15 August 2017, Pages 48-55