کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5131645 | 1491327 | 2017 | 9 صفحه PDF | دانلود رایگان |
- An antibody was analyzed using weak cation exchange chromatography at intact and subunit level after IdeS digestion.
- Modifications were identified by LC-MS analysis of intact and subunit molecular weights, and peptide mapping.
- The antibody with an succinimide from aspartate isomerization was identified as an acidic species.
An efficient strategy to characterize recombinant monoclonal antibody charge variants was established using weak anion exchange chromatography, LC-MS and IdeS digestion to allow subunit level characterization. Significantly higher resolution was achieved at subunit levels by weak anion exchange chromatography and LC-MS. In addition, subunit analysis localized potential modifications to either F(abâ²)2 or Fc fragments to facilitate further characterization. Peptide mapping of fractions from various charge variants after IdeS digestion identified aspartate isomerization, asparagine deamidation and glycation as the modifications. Although, aspartate isomerization does not generate net charge difference directly, it does generate antibody basic species. Antibodies with either isoaspartate or aspartate from deamidation showed different retention times by chromatography. Even more interestingly, the antibody contained succinimide as the isomerization intermediate, which though more basic compared to aspartate, eluted off the weak anion exchange column as an acidic species. The results demonstrated not only the utility of subunit level characterization but also the unpredictable chromatographic behavior of antibody charge variants.
Journal: Analytical Biochemistry - Volume 520, 1 March 2017, Pages 49-57