کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5131782 1491332 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Intrinsic protein fluorescence assays for GEF, GAP and post-translational modifications of small GTPases
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Intrinsic protein fluorescence assays for GEF, GAP and post-translational modifications of small GTPases
چکیده انگلیسی


- Intrinsic protein fluorescence - a quick and powerful tool to monitor GEF/GAP activity and post-translational modifications.
- Evidence suggests physiological and also pathological importance of post-translational modifications in the switch II region.
- Intrinsic fluorescence should be the first method chosen for monitoring such reactions of tryptophan-containing small GTPases.

Evidence and arguments are summarized that suggest that intrinsic (tryptophan) protein fluorescence provides an excellent and convenient signal for monitoring both GEF (guanine nucleotide exchange factor) and GAP (GTPase activating protein) activity of a large number of small GTPases. In addition, post-translational modifications of Rab proteins occurring in a region known to be a hot spot for such modifications also lead to fluorescence changes that can be accurately monitored in a time-dependent manner. It is suggested that intrinsic fluorescence should be the first method chosen for monitoring such reactions of tryptophan-containing small GTPases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 515, 15 December 2016, Pages 22-25
نویسندگان
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