کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5138912 1494816 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bioaccumulation and distribution of selenium in Enterococcus durans
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Bioaccumulation and distribution of selenium in Enterococcus durans
چکیده انگلیسی
Selenium is an essential nutrient for all living organisms. Under appropriate conditions lactic acid bacteria (LAB) are capable for accumulating large amounts of trace elements, such as selenium, and incorporating them into organic compounds. In this study, the capacity of selenium bioaccumulation by Enterococcus durans LAB18s was evaluated. The distribution of organic selenium in selenium-enriched E. durans LAB18s biomass was analyzed, and the highest percentage of organic selenium was found in the fraction of total protein, followed by the fractions of polysaccharides and nucleic acids. When the protein fraction was obtained by different extractions (water, NaCl, ethanol and NaOH) it was demonstrated that alkali-soluble protein showed the higher Selenium content. Analysis of protein fractions by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that selenium was present in the proteins ranging from 23 to 100 kDa. The cells were analyzed by scanning electron microscopy (SEM); scanning electron microscopy/energy dispersive spectrometry (SEM/EDS) and transmission electron microscopy (TEM). SEM, TEM and SEM/EDS showed the morphology, the selenium particles bioaccumulated into and on the cells and the amounts of selenium present into the cells, respectively. Thus, the isolate E. durans LAB18s can be a promising probiotic to be used as selenium-enriched biomass in feed trials.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Trace Elements in Medicine and Biology - Volume 40, March 2017, Pages 37-45
نویسندگان
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