کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5138997 | 1494861 | 2017 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A label-free electrochemical DNA sensor to identify breast cancer susceptibility
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A label-free electrochemical DNA sensor to identify breast cancer susceptibility A label-free electrochemical DNA sensor to identify breast cancer susceptibility](/preview/png/5138997.png)
چکیده انگلیسی
Detection of mutations related to cancer predisposition has been an important action in the prevention of cancer and in the cancer genetic profiling. Breast cancer (BRCA) genes mutations are widely known for their relation to genetic predisposition to breast and ovarian cancer. However, the access to genetic tests is still limited. Here, a label-free DNA sensor to detect the mutation 185delAG in the breast cancer 1, early onset (BRCA 1) gene, a founder mutation in the Ashkenazi population, is described. Based on the DNA hybridization and electrochemical impedance spectroscopy (EIS), the sensor was able to distinguish between the mutated and non-mutated sequences, a two-base mismatch. Using a single-strand thiol-modified 25-mer DNA sequence (HS-ssDNA) as probe immobilized in a gold electrode, hybridization studies were performed with [Fe(CN)6]4 â as redox probe and resulted in DNA sensor with a low limit of detection of 0.15 nmol Lâ 1 in a linear range from 1 to 200 nmol Lâ 1. This DNA sensor shows prominent electrochemical performance and might be an interesting alternative to distinguish between mutated and non-mutated BRCA1 genes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microchemical Journal - Volume 133, July 2017, Pages 37-42
Journal: Microchemical Journal - Volume 133, July 2017, Pages 37-42
نویسندگان
LaÃs Ribovski, Valtencir Zucolotto, Bruno C. Janegitz,