Conduritol F, the discriminant marker between C. wilfordii and C. auriculatum by 1H NMR spectroscopy

Quantitative 1H nuclear magnetic resonance (qNMR) spectroscopy is a powerful and versatile technique to enable the absolute quantification of specific components in a mixture with excellent reproducibility and robustness. In the present study, qNMR analysis was applied to conduritol F, a chemical marker with the potential to distinguish between C. wilfordii and C. auriculatum. We found that the signals of H-5 and H-6 of conduritol F were well-separated from others with purities sufficient to be used to distinguish between C. wilfordii and C. auriculatum. This simple methodology can be applied to identify one or two species in products or powdered herbs widely distributed in the markets.