Chemical constituents of Carissa edulis Vahl

Reinvestigation of the biologically active ethyl acetate and butanol extracts of the aerial parts of Carissa edulis afforded 3-O-acetyl chlorogenic acid (I), along with four known flavonol glucosides including, kaempferol 3-O-β-d glucopyranoside (II), quercetin-3-O-β-d glucopyranoside (III), rhamnetin-3-O-β-d glucopyranoside (IV) and isorhamnetin-3-O-β-d-glucopyranoside (V) from ethyl acetate fraction. Isorhamnetin-3-O-β-d-glucopyranoside-(2″→1″′)-rhamnopyranoside (VI), Caredulis, 1-{1-[2-(2 hydroxypropoxy) propoxy] propan-2-yloxy} propan-2-ol (VII) and (+) butyl-O-α-l-rhamnoside (VIII) were isolated from butanol fraction. Characterization of these compounds was achieved by various spectroscopic methods (UV, MS, 1H NMR, 13C NMR, COSY, HSQC and HMBC) and through comparison with published data. Compounds I-VIII were isolated from C. edulis for the first time, while compounds VII and VIII were isolated for the first time from nature.