Human Mesenchymal Stem Cells Expansion on Three-Dimensional (3D) Printed Poly-Styrene (PS) Scaffolds in a Perfusion Bioreactor

In this study, we present a novel approach of expanding Adipose derived hMSCs (human Mesenchymal Stem Cells) seeded on 3D printed Poly-Styrene (PS) scaffolds within a perfusion based cell expansion bioreactor. 3D printed PS scaffolds inoculated with adipose derived hMSCs were cultured statically for 9 days in 2D for assuring the viability and proliferation expansion of hMSCs on PS scaffolds. Thereafter, a batch of pre-seeded scaffolds were dynamically cultured inside a perfusion based bioreactor system for 8 days to assess the expansion of hMSCs on the PS scaffolds. As compared to static 3D culture (control), the 3D perfusion based system resulted in comparable fold-expansion and viability. Osteogenic and Adipogenic differentiation potential of the harvested cells were evaluated to assess if the stem cells retained their stem cell properties at the end of the expansion step.