کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5503229 | 1535087 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The antiepileptogenic effect of low-frequency stimulation on perforant path kindling involves changes in regulators of G-protein signaling in rat
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کلمات کلیدی
RGSLFSKLFsregulators of G-protein signalingpEPSPcAMP - cAMPCyclic adenosine monophosphate - آدنوزین مونوفسفات CyclicADD - اضافه کردنPopulation spike - افزایش جمعیتlow frequency stimulation - تحریک فرکانس پایینBrain stimulation - تحریک مغزیseizure - تشنج long term potentiation - تقویت طولانی مدتLTP - تقویت طولانی مدت یا LTP dentate gyrus - شکنج دندانه دارafterdischarge duration - مدت زمان پس از تخلیه
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The antiepileptogenic effect of low-frequency stimulation on perforant path kindling involves changes in regulators of G-protein signaling in rat The antiepileptogenic effect of low-frequency stimulation on perforant path kindling involves changes in regulators of G-protein signaling in rat](/preview/png/5503229.png)
چکیده انگلیسی
G-protein coupled receptors may have a role in mediating the antiepileptogenic effect of low-frequency stimulation (LFS) on kindling acquisition. This effect is accompanied by changes at the intracellular level of cAMP. In the present study, the effect of rolipram as a phosphodiesterase inhibitor on the antiepileptogenic effect of LFS was investigated. Meanwhile, the expression of αs- and αi-subunit of G proteins and regulators of G-protein signaling (RGS) proteins following LFS application was measured. Male Wistar rats were kindled by perforant path stimulation in a semi-rapid kindling manner (12 stimulations per day) during a period of 6 days. Application of LFS (0.1 ms pulse duration at 1 Hz, 200 pulses, 50-150 μA, 5 min after termination of daily kindling stimulations) to the perforant path retarded the kindling development and prevented the kindling-induced potentiation and kindling-induced changes in paired pulse indices in the dentate gyrus. Intra-cerebroventricular microinjection of rolipram (0.25 μM) partially prevented these LFS effects. Twenty-four hours after the last kindling stimulation, the dentate gyrus was removed and changes in protein expression were measured by Western blotting. There was no significant difference in the expression of α-subunit of Gs and Gi/o proteins in different experimental groups. However, application of LFS during the kindling procedure decreased the expression RGS4 and RGS10 proteins (that reduce the activity of Gi/o) and prevented the kindling-induced decrease of RGS2 protein (which reduces the Gs activity). Therefore, it can be postulated that the Gi/o protein signaling pathways may be involved in antiepileptogenetic effect of LFS, and this is why decreasing the cAMP metabolism by rolipram attenuates this effect of LFS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the Neurological Sciences - Volume 375, 15 April 2017, Pages 450-459
Journal: Journal of the Neurological Sciences - Volume 375, 15 April 2017, Pages 450-459
نویسندگان
Simin Namvar, Yaghoub Fathollahi, Mohammad Javan, Maryam Zeraati, Mohammad Mohammad-Zadeh, Amir Shojaei, Javad Mirnajafi-Zadeh,