کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5507113 | 1536899 | 2017 | 5 صفحه PDF | دانلود رایگان |
- Nano-silica particles increased cytosolic calcium flux in fibroblasts.
- Calcium flux by nano-SPs was suppressed by SKF96365 and thapsigargin.
- Calcium flux modulation by nano-SPs was determined by their surface structure.
Several studies have reported that amorphous nano-silica particles (nano-SPs) modulate calcium flux, although the mechanism remains incompletely understood. We thus analyzed the relationship between calcium flux and particle surface properties and determined the calcium flux route. Treatment of Balb/c 3T3 fibroblasts with nano-SPs with a diameter of 70Â nm (nSP70) increased cytosolic calcium concentration, but that with SPs with a diameter of 300 or 1000Â nm did not. Surface modification of nSP70 with a carboxy group also did not modulate calcium flux. Pretreatment with a general calcium entry blocker almost completely suppressed calcium flux by nSP70. Preconditioning by emptying the endoplasmic reticulum (ER) calcium stores slightly suppressed calcium flux by nSP70. These results indicate that nSP70 mainly modulates calcium flux across plasma membrane calcium channels, with subsequent activation of the ER calcium pump, and that the potential of calcium flux by nano-SPs is determined by the particle surface charge.
Journal: Biochemistry and Biophysics Reports - Volume 9, March 2017, Pages 330-334