کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5517055 | 1543038 | 2017 | 5 صفحه PDF | دانلود رایگان |
- Monoclonal antibodies were engineered against recombinant ISG (rISG) of T. evansi.
- An inhibition immunoassay exploring MAbs and rISG was developed to diagnose surra.
- 98.8% diagnostic sensitivity and 99.2% specificity were found.
- A Panel of 1192 serum samples was tested with CATT and new assay for comparison.
- Chi-square and kappa value revealed agreement between developed test and CATT.
The present study is aimed at the development of inhibition ELISA (I-ELISA) exploring monoclonal antibodies (MAbs) and recombinant invariant surface glycoprotein. The extracellular domain (ED) of invariant surface glycoprotein (ISG-75) from Trypanosoma evasni has been heterologously expressed in Pichia pastoris (X-33). The recombinant ISG-75 (rISG-75ED) was characterized by immunoblot and ELISA, followed by the production of MAbs against rISG-75ED. The MAbs were characterized by immunoblot and then explored in the development of I-ELISA for the detection of surra. The diagnostic potential of the developed test has been evaluated using 1192 field sera sample including cattle, buffalo, donkey, horse and camel. The statistical analysis of the data showed optimum combination of diagnostic sensitivity and specificity at 98.8% and 99.2% respectively, with cut-off percentage inhibition (PI) value of >45. The Cohen's kappa coefficient of agreement was found to be 0.98. Hence, the diagnostic test developed in the present study can be exploited as a potential and reliable tool in the serodiagnosis and surveillance of surra in animals.
Journal: Biologicals - Volume 46, March 2017, Pages 148-152