کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5519336 | 1544101 | 2017 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Contryphan-Bt: A pyroglutamic acid containing conopeptide isolated from the venom of Conus betulinus Contryphan-Bt: A pyroglutamic acid containing conopeptide isolated from the venom of Conus betulinus](/preview/png/5519336.png)
- A new member of the contryphan family, contryphan-Bt was isolated from the venom of Conus betilinus.
- The sequence of contryphan-Bt is ascertained as ZSGCO(D-W)KPWC-NH2 (Z, pyroglutamic acid).
- This finding is the first instance of the identification of pyroglutamic acid in contryphan.
A new member of the contryphans family was isolated from the venom of Conus betilinus, a vermivorous species distributed in the South China Sea. Its sequence, ZSGCO(D-W)KPWC-NH2 (Z, pyroglutamic acid), was established by a combination of de novo MS/MS sequencing and venom-duct transcriptome sequencing. The occurrence of D-Trp6 was confirmed by chemical synthesis and HPLC behavior comparison. Like known contryphans, contryphan-Bt produces the “stiff-tail” syndrome in mice and contains one disulfide bond, a hydroxyproline, a D-tryptophan, and an amidated C-terminus. However, contryphan-Bt differs from previously identified contryphans by a pyroglutamic acid at the N terminus. CD spectrum reveals that contryphan-Bt possess β-turn in solution.
Journal: Toxicon - Volume 135, 1 September 2017, Pages 17-23