کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5522588 1546028 2017 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Generation of human erythroblast-derived iPSC line using episomal reprogramming system
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Generation of human erythroblast-derived iPSC line using episomal reprogramming system
چکیده انگلیسی

Peripheral blood mononuclear cells were isolated and cultured to a pure pro-EBL population and reprogrammed using episomal plasmids. The pluripotency of transgene-free induced pluripotent stem cell (iPSC) line was verified by the expression of pluripotency-associated markers and by in vitro spontaneous differentiation towards the 3 germ layers. The iPSC line showed normal karyotype. Peripheral blood is a non-invasive easy accessible cell source and combined with EBL outgrowth in vitro, a routine process obtaining sufficient amount of homogenous cells can be obtained within a week. Using episomal delivery, pro-EBLs can be reprogrammed in a transgene-free, cost effective system.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Stem Cell Research - Volume 25, December 2017, Pages 30-33
نویسندگان
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