کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5522649 1546032 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
P-Cadherin is necessary for retinal stem cell behavior in vitro, but not in vivo
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
P-Cadherin is necessary for retinal stem cell behavior in vitro, but not in vivo
چکیده انگلیسی


- Retinal stem cells are rare pigmented cells in the pigmented ciliary epithelium that express P-Cadherin.
- P-Cadherin is required for the initiation of sphere formation in vitro.
- Both P- and N-Cadherin are required for the formation of the retinal stem cell spheres in vitro.
- P-Cadherin is not required for retinal stem cell maintenance in vivo, and may be compensated for by other adhesion mechanisms.

Adult retinal stem cells (RSCs) are rare quiescent cells within the ciliary epithelium of the eye, which is made up of non-pigmented N-Cadherin+ ve inner and pigmented P-Cadherin+ ve outer cell layers. Through FACs and single cell analyses, we have shown that RSCs arise from single cells from within the pigmented CE and express P-Cadherin. However, whether the expression of P-Cadherin is required for maintenance of the stem cell in vivo or in the formation of the clonal stem cell spheres in vitro is not known. Using cadherin functional blocking antibody experiments and a P-Cadherin −/− mouse to test whether the RSC population is affected by the loss of P-Cadherin expression, our experiments demonstrate that the RSCs reside in the pigmented CE layer and express P-Cadherin, which is important to the formation of adherent sphere colonies in vitro, however P-Cadherin is not required for maintenance of RSCs in vivo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Stem Cell Research - Volume 21, May 2017, Pages 141-147
نویسندگان
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