کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5522732 | 1546034 | 2017 | 10 صفحه PDF | دانلود رایگان |
- Global promoter CpG methylation controls hESC HLA-G mRNA expression.
- hESC show a specific epi-allele with a low methylation grade in region -121 to -188.
- HLA-G 3â²UTR genotype regulates HLA-G mRNA expression in hESC.
- No influence of miRNA expression level on HLA-G expression
The human leukocyte antigen (HLA)-G gene seems to play a pivotal role in maternal tolerance to the fetus. Little is known about HLA-G expression and its molecular control during in vivo human embryogenesis. Human embryonic stem cells (hESC) provide an interesting in vitro model to study early human development. Different studies reported discrepant findings on whether HLA-G mRNA and protein are present or absent in hESC. Several lines of evidence indicate that promoter CpG methylation and 3â² untranslated region (3â²UTR) polymorphisms may influence HLA-G expression.We investigated how HLA-G expression is linked to the patterns of promoter methylation and explored the role of the 3â²UTR polymorphic sites and their binding microRNAs on the post-transcriptional regulation of HLA-G in eight hESC lines.We showed that, while the gross expression levels of HLA-G are controlled by promoter methylation, the genetic constitution of the HLA-G 3â²UTR, more specifically the 14bp insertion in combination with the +Â 3187A/A and +Â 3142G/G SNP, plays a major role in HLA-G mRNA regulation in hESC.Our findings provide a solid first step towards future work using hESC as tools for the study of early human developmental processes in normal and pregnancy-related disorders such as preeclampsia.
Journal: Stem Cell Research - Volume 19, March 2017, Pages 118-127