کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5522815 1546031 2017 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9
چکیده انگلیسی

SOX2 is an important transcription factor involved in pluripotency maintenance, pluripotent reprogramming and differentiation towards neural lineages. Here we engineered the previously described HEL24.3 hiPSC to generate a SOX2 reporter by knocking-in a T2A fused nuclear tdTomato reporter cassette before the STOP codon of the SOX2 gene coding sequence. CRISPR/SaCas9-mediated stimulation of homologous recombination was utilized to facilitate faithful targeted insertion. This line accurately reports the expression of endogenous SOX2 and therefore constitutes a useful tool to study the SOX2 expression dynamics upon hiPSC culture, differentiation and somatic cell reprogramming.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Stem Cell Research - Volume 22, July 2017, Pages 16–19