کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5522827 | 1546035 | 2017 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Derivation and characterization of the human embryonic stem cell line CR-4: Differentiation to human retinal pigment epithelial cells
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Derivation and characterization of the human embryonic stem cell line CR-4: Differentiation to human retinal pigment epithelial cells Derivation and characterization of the human embryonic stem cell line CR-4: Differentiation to human retinal pigment epithelial cells](/preview/png/5522827.png)
چکیده انگلیسی
The CR-4 human embryonic stem cell line was derived from the inner cell mass of a developing blastocyst. This cell line has been adapted to grow in feeder-free conditions and is especially well-suited for differentiation to retinal pigment epithelium. The line demonstrates a normal human 46,XX female karyotype. Pluripotency was assessed through qRT-PCR for expression of NANOG, OCT-4, and SOX-2. A teratoma assay was performed and results were positive for all three germ layers. Testing for Mycoplasma was negative.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Stem Cell Research - Volume 18, January 2017, Pages 37-40
Journal: Stem Cell Research - Volume 18, January 2017, Pages 37-40
نویسندگان
John L. Mazzilli, Aleksey Y. Domozhirov, Stacey L. Mueller-Ortiz, Charles A. Garcia, Rick A. Wetsel, Eva M. Zsigmond,