کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5522827 1546035 2017 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Derivation and characterization of the human embryonic stem cell line CR-4: Differentiation to human retinal pigment epithelial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Derivation and characterization of the human embryonic stem cell line CR-4: Differentiation to human retinal pigment epithelial cells
چکیده انگلیسی

The CR-4 human embryonic stem cell line was derived from the inner cell mass of a developing blastocyst. This cell line has been adapted to grow in feeder-free conditions and is especially well-suited for differentiation to retinal pigment epithelium. The line demonstrates a normal human 46,XX female karyotype. Pluripotency was assessed through qRT-PCR for expression of NANOG, OCT-4, and SOX-2. A teratoma assay was performed and results were positive for all three germ layers. Testing for Mycoplasma was negative.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Stem Cell Research - Volume 18, January 2017, Pages 37-40
نویسندگان
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