کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5528696 1548307 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sample size determination for the fluctuation experiment
ترجمه فارسی عنوان
تعیین حجم نمونه برای آزمایش نوسان
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
چکیده انگلیسی


- Sample size determination for the fluctuation experiment so far relies on intuition.
- The expected Fisher information can be utilized to help determine sample size.
- Two criteria are suggested to guide the determination of sample size.
- An explicit formula is given to calculate the required sample size.

The Luria-Delbrück fluctuation experiment protocol is increasingly employed to determine microbial mutation rates in the laboratory. An important question raised at the planning stage is “How many cultures are needed?” For over 70 years sample sizes have been determined either by intuition or by following published examples where sample sizes were chosen intuitively. This paper proposes a practical method for determining the sample size. The proposed method relies on existing algorithms for computing the expected Fisher information under two commonly used mutant distributions. The role of partial plating in reducing sample size is discussed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Volume 795, January 2017, Pages 10-14
نویسندگان
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