کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5528851 1548560 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The Vitotox and ToxTracker assays: A two-test combination for quick and reliable assessment of genotoxic hazards
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
The Vitotox and ToxTracker assays: A two-test combination for quick and reliable assessment of genotoxic hazards
چکیده انگلیسی


- The performance of Vitotox and Toxtracker is tested for genotoxicity assessment.
- Reference compounds cover broad applicability domain and/or mechanism of action.
- Both reporter-based assays are reliable tools especially when used as a test battery.

To ensure safety for humans, it is essential to characterize the genotoxic potential of new chemical entities, such as pharmaceutical and cosmetic substances. In a first tier, a battery of in vitro tests is recommended by international regulatory agencies. However, these tests suffer from inadequate specificity: compounds may be wrongly categorized as genotoxic, resulting in unnecessary, time-consuming, and expensive in vivo follow-up testing. In the last decade, novel assays (notably, reporter-based assays) have been developed in an attempt to overcome these drawbacks. Here, we have investigated the performance of two in vitro reporter-based assays, Vitotox and ToxTracker. A set of reference compounds was selected to span a variety of mechanisms of genotoxic action and applicability domains (e.g., pharmaceutical and cosmetic ingredients). Combining the performance of the two assays, we achieved 93% sensitivity and 79% specificity for prediction of gentoxicity for this set of compounds. Both assays permit quick high-throughput analysis of drug candidates, while requiring only small quantities of the test substances. Our study shows that these two assays, when combined, can be a reliable method for assessment of genotoxicity hazard.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 810, 1 November 2016, Pages 13-21
نویسندگان
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