کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5543113 | 1553930 | 2017 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The multiple adrenocorticotropic hormone injections significantly alters hepatic proteome in growing pigs
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Currently, the effects of stress on the body metabolism are attracting more and more attentions. To better understand the probable stress-induced metabolic changes, the changes in plasma indices and hepatic proteome were investigated in pigs fitted with jugular venous catheters administered adrenocorticotropic hormone (ACTH), intramuscularly twice daily for 7 days. In particular, plasma hormones, blood cells and biochemical indicators were analyzed. In addition, the hepatic proteome was analyzed by two-dimensional electrophoresis and matrix-assisted laser desorption ionization time of flight mass spectrometry. The results revealed that after the initial ACTH injection, plasma ACTH, cortisol, triiodothyronine and tumor necrosis factor α increased (P Ë 0.05). Additionally, lactic dehydrogenase activity increased (P < 0.05), and both leukocyte and lymphocyte count decreased (P < 0.05). In comparison, after the ACTH injection for 7 days, hepatic proteomics analysis identified 27 significantly changed proteins and most of these proteins (49%) are involved in metabolism, but plasma indices showed no obvious change. In conclusion, compared with the obvious changes in plasma indices after the initial ACTH injection, the multiple ACTH injections did not change plasma indices, yet the hepatic proteome profile was significantly altered. The results provide useful information for a better understanding of the effects of stress on metabolism.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Livestock Science - Volume 202, August 2017, Pages 180-187
Journal: Livestock Science - Volume 202, August 2017, Pages 180-187
نویسندگان
Jun Guo, Chao Yin, Shixing Gao, Weifeng Liu, Yanping Huang, Ruqian Zhao, Xiaojing Yang,